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Photostable green nucleic stain for auto-fluorescence free imaging – TREM-green

The TREM-Green photostable green nucleic stain is a brightly emissive, green nuclear and cellular stain that is useful for imaging of live cells, fixed cells and fixed tissue.  It is a photostable platinum(II) complex that quickly accumulates in cells and can provide autofluorescence-free imaging of various live eukaryotic cell types.

Features of TREM-green include:

  • High quantum yield
  • Photobleaching resistance (photostable)
  • Excitation: 405 nm or 760-900 nm
  • Suitable for multi-photon imaging
  • Live cell imaging

Emission from this small molecule agent lasts for microseconds, 1000 times longer than both the autofluorescence of cells and the fluorescence of the conventional organic agents. This allows time-gated experiments to cut out autofluorescence from cells or tissue samples.

TREM-green is resistant to photobleaching.  It’s exceptional photostability makes it particularly suitable for Time-Resolved Emission Imaging Microscopy (TREM) as well as two-photon/multiphoton imaging, depth imaging, phosphorescence lifetime imaging (PLIM) and fluorescence lifetime imaging.

Excitation frequencies

The stain has a quantum yield of 70%, can be excited by 405 nm in confocal imaging and by 760 – 900 nm light in multiphoton imaging

Other advantages

TREM-green has the added advantage of being strongly oxygen sensitive and can therefore be used as oxygen sensor, oxygen probe in-cellulo or in tissues.

As a green photostable stain, it is perfectly suited for use with blue and red fluorophores in multicolour experiments.

Customers can purchase TREM-green directly from Redbrick Molecular.  To place an order, please use the contact us link below.

Instructions for use

TREM-green is simply dissolved in DMSO to make a stock solution that can be stored for multiple months in the freezer.  Live cells are incubated with the solution for 5 minutes before being washed and imaged.  Please click on the documents tab above to download a protocol for use.

Supported by publication

The platinum complex, TREM-green, has been used in a number of academic publications e.g. Time-resolved and two-photon emission imaging microscopy of live cells with inert platinum complexes.  Please see the documents tab for further references.

WeightPrice (GBP)
Supporting Documents
Specification Sheet
TREM Green Protocol
Botchway SW et al, Time-resolved and two-photon emission imaging microscopy of live cells with inert platinum complexes - Proc Natl Acad Sci U S A. 2008 Oct 21;105(42):16071-6. doi: 10.1073/pnas.0804071105.
Julia A. Weinstein et al, Long-lived metal complexes open up microsecond lifetime imaging microscopy under multiphoton excitation: from FLIM to PLIM and beyond, Chem. Sci., 2014,5, 879-886
Julia A. Weinstein et al, Oxygen Mapping of Melanoma Spheroids using Small Molecule Platinum Probe and Phosphorescence Lifetime Imaging Microscopy, Scientific Reportsvolume 7, Article number: 10743 (2017) doi:10.1038/s41598-017-11153-9