Photostable green nucleic stain for auto-fluorescence free imaging – TREM-green
The TREM-Green photostable green nucleic stain is a brightly emissive, green nuclear and cellular stain that is useful for imaging of live cells, fixed cells and fixed tissue. It is a photostable platinum(II) complex that quickly accumulates in cells and can provide autofluorescence-free imaging of various live eukaryotic cell types.
Features of TREM-green include:
- High quantum yield
- Photobleaching resistance (photostable)
- Excitation: 405 nm or 760-900 nm
- Suitable for multi-photon imaging
- Live cell imaging
Emission from this small molecule agent lasts for microseconds, 1000 times longer than both the autofluorescence of cells and the fluorescence of the conventional organic agents. This allows time-gated experiments to cut out autofluorescence from cells or tissue samples.
TREM-green is resistant to photobleaching. It’s exceptional photostability makes it particularly suitable for Time-Resolved Emission Imaging Microscopy (TREM) as well as two-photon/multiphoton imaging, depth imaging, phosphorescence lifetime imaging (PLIM) and fluorescence lifetime imaging.
The stain has a quantum yield of 70%, can be excited by 405 nm in confocal imaging and by 760 – 900 nm light in multiphoton imaging
TREM-green has the added advantage of being strongly oxygen sensitive and can therefore be used as oxygen sensor, oxygen probe in-cellulo or in tissues.
As a green photostable stain, it is perfectly suited for use with blue and red fluorophores in multicolour experiments.
Customers can purchase TREM-green directly from Redbrick Molecular. To place an order, please use the contact us link below.
Instructions for use
TREM-green is simply dissolved in DMSO to make a stock solution that can be stored for multiple months in the freezer. Live cells are incubated with the solution for 5 minutes before being washed and imaged. Please click on the documents tab above to download a protocol for use.
Supported by publication
The platinum complex, TREM-green, has been used in a number of academic publications e.g. Time-resolved and two-photon emission imaging microscopy of live cells with inert platinum complexes. Please see the documents tab for further references.